Materials And Methods For Afm Adhesion Measurements

4. Materials and Methods for AFM Adhesion Measurements 4.1. Materials Paraformaldehyde (4% in 0.1 M Phosphate Buffer Saline), 1 Phosphate Buffered Saline (PBS) and Muscovite Mica (V-1 Quality) were purchased from Electron Microscopy Sciences (Hatfield, PA). Coverslips (No.1.5 thickness) were purchased from MatTek Corporation (Ashland, MA). MDA-MB-231 and Hs578Bst cell lines, growth media (L-15 fand 46-X, respectively), and media supplements (penicillin/streptomycin and fetal bovine serum) were purchased from American Type Culture Collection (ATCC, Manassas, VA). Uncoated n-doped silicon AFM probes (MPP-31100) were purchased from Bruker (Billerica, MA). 4.2. AFM Tip Preparation AFM tips were functionalized using a simple†¦show more content†¦The breast cancer cells were incubated at 37  °C under normal atmospheric pressure, while the normal breast cancer cells were incubated at 37  °C in environments with 5% CO2 levels, respectively. The MDA-MB-231 and Hs578bst cells were grown respectively in L-15 or 46-X base media. These were supplemented with 100 I.U./ml penicillin/100 ÃŽ ¼g/ml streptomycin and 10% FBS. The cells were first rinsed with 1X PBS and then fixed with 4 wt% paraformaldehyde solution for 10 mins at room temperature. They were then rinsed with PBS solution for three more times, before a final rinse with purified water. Finally, the samples were dried in a vacuum desiccator for at least 5 hours. 4.4. AFM tip Characterization An FEI Quanta 200 FEG Environmental-SEM (FEI, Hillsboro, OR) was used to view the AFM tips before and after coating. This was done to determine whether or not the AFM tips were successfully dip-coated. The tips were also viewed in the ESEM after the pull-off tests. This was done to ensure that no coating was lost during the AFM pull-off. 4.5. Force-Displacement Measurements All of the AFM experiments were performed in a Dimension NanoMan AFM (Bruker, Billerica, MA). This was done under ambient conditions at room temperature of 24  °C and a relative humidity of 45%. The photo-detector sensitivity was calibrated on a hard mica surface. The adhesive forces were measured for the following configurations: (1) bare tips and normal

Personal Narrative The Cold Bucket Challenge - 1106 Words

Lying in bed, with the blankets tucked tightly around my head. Sleeping in a deep comatose, suddenly the alarm went off; squealing like my Company Commander woke us up. I quickly reached for the alarm clock and brought it down, towards my face, so I could attempt to see what time it was. I finally snapped out of my sleep induced haze, as if someone had dumped a bucket of cold water over my head, just like they were doing in the â€Å"Cold Bucket Challenge.† I thought to myself that I really had to out of my warm bed, to go pick up Jessica up from work. Quietly I laid there dreading getting up from the warm nest that I had built. Finally I cleared the cobwebs out of my sleep driven mind. And threw my legs over the side of the bed, standing there reaching for my glasses. I quickly got dressed, and opened up the curtain to take a look outside. As I stood there focusing my eyes. I snapped back into reality. I noticed that it had begun to snow, and man was it snowing har d. Shaking, my head, in disbelief, I walked into the kitchen to grab the broom. Stopping at the closet, I grabbed my coat and gloves. I thought to myself that this is could get real ugly. Carefully I opened the door, uncertain of what I was going to see. A cold blast of arctic air hit me, as if I stuck my head in a deep freezer. I carefully walked through the drifts, to get to the car. I walked extremely carefully so I wouldn’t take a nose dive into a friendly snowbank. Carefully I began to use theShow MoreRelatedStudy Guide Literary Terms7657 Words   |  31 PagesFlies provides a compelling allegory of human nature, illustrating the three sides of the psyche through its sharply-defined main characters. A form of extended metaphor, in which objects, persons, and actions in a narrative, are equated with the meanings that lie outside the narrative itself. 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Directing Act 5 Scene 2 Essay Example For Students

Directing Act 5 Scene 2 Essay I will be directing part of Act five scene two. This is the part where most of the main characters are all together and Petruchio shows all the other men that he has tamed Kate. All the men have a bet on whose wife is the most obedient.  I have chosen to set my piece in the present time because I want the modern day audience to sympathise with both Katherina and Petruchio. I am going to set this in a stately home because the men will be playing cards and the women will be talking in a nearby room. The servants will be also playing cards because Biondello and Gremio are also good friends of their masters. The house will belong to Lucentio. I have chose to use a stately home because it is the closest thing to the type of housing and the class the people in the original play were set in. I thought that although the stately home is the closest thing to the original, the modern day audience would still be able to relate to the characters and settings a lot easier. The men will be dressed in casual suits with a loosened tie, to create the effect of the upper class relaxing with a few friends. Kate will be wearing a long flowing dress to show that she is like an ordinary upper class lady instead of the more radical clothing she would have worn before the taming process. Biannca and the widow will also be wearing the same style of dress. The servants (Biondello and Gremio) would be wearing the traditional butler uniform.  I want them to realise that Petruchio has tamed Kate, but I want them to question whether or not Kate has lost her robust personality in the process. I want them to think that although Kate has been disciplined and is obeying Petruchios every word, that she still is her own person, but she now puts her view across in very different ways. I am going to direct my play in a way that will bring out the fact that Kate is still her own person, but a lot more sociable.  Baptista and all the other men think that Petruchio could not have tamed Kate because she was uncontrollable. So I would get Baptista to say this line   Now in good. shrew of all  In a patronising way. I would get him to say it in this way because Baptista doesnt think that Petruchio has succeeded.  Well, I say no. we will propose  Petruchio would say this in a confident manner, which the other gentlemen will act upon because they think that Kate has no chance of winning therefore they all place a wager for their own wife. Twenty crowns? Ill venture. Upon my wife  Again Petruchio would say this in a conclusive way to make lure the audience and his friends into thinking he must be foolish to bet such a large amount of money upon a bet he is in no doubt going to lose.  That will I. Go Biondello, bid your mistress come to me  Lucentio would say this in a boastful manner because he thinks his sweet fair Biannca will come to him at once. The audience are in total agreement with Lucentio, they also think Biannca will come. When Biondello exits he will go through a large oak door and you would hear him running down the hall. Son, Ill be your half Biannca comes  The actor would need to say this in a way that convinces the audience he is willing to give his money away because he is that assured that Lucentio will win the bet.  Ill have no halves; Ill bear it all myself.  Lucentio would say this in an arrogant way because he is that confident that he will win he wants all the rewards for himself.  Sir, my mistress. She cannot come.  Biondello would be out of breath after he had been running; he would then say this sentence in a very surprised way. Before he said this Lucentio would have a very smug grin on his face, but afterwards his face would drop.  How? Shes busy. Is that an answer?

Company Accounting for ASX Corporate Governance Principles

Question: Discuss about theCompany Accounting for ASX Corporate Governance Principles. Answer: Australias corporate governance and continuous disclosure framework is regarded the best in the world. This is the reason why it has also been adopted by various other jurisdictions. Moreover, the Corporations Law in Australia require organizations to keep the market informed of significant developments and there are negative consequences for those who fail. In relation to Getswift Ltd, it is notable that the acquisition of Pursuit Pty Ltd necessitates variations in its current corporate governance practices so that it can easily adhere to the ASX Corporate Governance Principles. In relation to structuring the Board of the company, it is notable that there are no committees established for the purpose of discharge of responsibilities. Furthermore, since acquisition of Pursuit Pty Ltd is a major task for Getswift, it is recommended that it facilitates proper provisions for the establishment of relevant committees like the nomination committee. The reason why establishment of such committee is crucial for the company can be attributed to the fact that it assists in examining the selection and appointment approaches of the company[1]. Furthermore, such committee must comprise of only non-executive directors who are responsible for reviewing Board succession measures, assessing performance of the Board and directors, and evaluating required and desirable competencies of such board members. Overall, with such recommendation, the company can be easily assisted in discharging its responsibilities in an effective way. Another crucial recommendation for the company in relation to compliance with the ASX Corporate Governance Principles is that it must make proper provisions for establishing an audit committee. Since the company has not designed an audit committee and it is overseen by the Board, it must be noted that integrity of financial reporting can only be attained through an audit committee[2]. Without an audit committee, Getswift cannot facilitate good corporate governance measure in the entire industry and may fail to achieve penetration into the market even by acquiring its major customer Pursuit Ltd. Furthermore, in relation to this, it must be noted that such establishment of audit committee must also have their own charter wherein the roles and responsibilities of committee members are clearly laid down. Moreover, if integrity of financial reporting is obtained through establishment of an audit committee, the company can easily comply with ASX Corporate Governance Framework[3]. Besides, such integrity is demanded by every stakeholder in the market and if Getswift does not consider such recommendation, it may fail to retain trust and confidence on the part of such stakeholders. The third recommendation to Getswift Ltd is that it must establish an efficient remuneration committee in its framework so that it can adhere to the requirements of ASX Corporate Governance Principles. The reason behind this can be attributed to the fact that the scale and size of the company has restricted it to frame a remuneration committee that will result in future complications after acquisition of Pursuit Ltd. Moreover, the roles and responsibilities of such committee are overseen by the Board of Getswift itself, thereby creating a scenario wherein it is becoming overburdened in nature. In relation to this, it is recommended to the company that it must design a proper remuneration committee so that the policies associated to remuneration are focused upon in an effective way. Besides, such committee must primarily comprise of non-executive directors of the company wherein majority are independent in nature[4]. However, if the board can manage the roles and responsibilities of t he Group, it can do so but there are various guidelines in relation to remuneration of executives and non-executive directors. In relation to this, it is observable that the company offers remuneration to its non-executive directors based on the level of remuneration paid to non-executive directors by organizations of similar size in the industry[5]. It is recommended that the company must remunerate its non-executive directors normally in the form of cash, superannuation contributions, non-cash benefits, or equity. Furthermore, such directors must not receive bonus payments or options by the Board. In addition, these directors must not be offered retirement benefits except for superannuation advantages. Therefore, the company must consider this recommendation of overseeing its fair and responsible strategy of remuneration for complying the objectives or requirements of ASX Corporate Governance Principles. Therefore, these recommendations can be taken into consideration that can allow Getswift comply with ASX requirements and effectively acquire its primary consumer named Pursuit Pty Ltd. Bibliography Fen Osler, Hampson. Turbulent Peace: the Challenges of Managing International Conflict, Washington, 2012. Kowalewski, Oskar. Corporate governance and corporate performance: financial crisis. Management Research Review 39, no. 1 (2016): 1494-1515. doi: 10.1108/MRR-12-2014-0287 Marc, Goergen. International Corporate Governance, Prentice Hall, 2012. Rachael Ajomboh, Ntongho,.Culture and corporate governance convergence. International Journal of Law and Management 58, no. 5 (2016): 523-544. doi: 10.1108/IJLMA-04-2015-0016 Thomas, Clarke. International Corporate Governance, New York: Routledge, 2010.