Materials And Methods For Afm Adhesion Measurements

4. Materials and Methods for AFM Adhesion Measurements 4.1. Materials Paraformaldehyde (4% in 0.1 M Phosphate Buffer Saline), 1 Phosphate Buffered Saline (PBS) and Muscovite Mica (V-1 Quality) were purchased from Electron Microscopy Sciences (Hatfield, PA). Coverslips (No.1.5 thickness) were purchased from MatTek Corporation (Ashland, MA). MDA-MB-231 and Hs578Bst cell lines, growth media (L-15 fand 46-X, respectively), and media supplements (penicillin/streptomycin and fetal bovine serum) were purchased from American Type Culture Collection (ATCC, Manassas, VA). Uncoated n-doped silicon AFM probes (MPP-31100) were purchased from Bruker (Billerica, MA). 4.2. AFM Tip Preparation AFM tips were functionalized using a simple†¦show more content†¦The breast cancer cells were incubated at 37  °C under normal atmospheric pressure, while the normal breast cancer cells were incubated at 37  °C in environments with 5% CO2 levels, respectively. The MDA-MB-231 and Hs578bst cells were grown respectively in L-15 or 46-X base media. These were supplemented with 100 I.U./ml penicillin/100 ÃŽ ¼g/ml streptomycin and 10% FBS. The cells were first rinsed with 1X PBS and then fixed with 4 wt% paraformaldehyde solution for 10 mins at room temperature. They were then rinsed with PBS solution for three more times, before a final rinse with purified water. Finally, the samples were dried in a vacuum desiccator for at least 5 hours. 4.4. AFM tip Characterization An FEI Quanta 200 FEG Environmental-SEM (FEI, Hillsboro, OR) was used to view the AFM tips before and after coating. This was done to determine whether or not the AFM tips were successfully dip-coated. The tips were also viewed in the ESEM after the pull-off tests. This was done to ensure that no coating was lost during the AFM pull-off. 4.5. Force-Displacement Measurements All of the AFM experiments were performed in a Dimension NanoMan AFM (Bruker, Billerica, MA). This was done under ambient conditions at room temperature of 24  °C and a relative humidity of 45%. The photo-detector sensitivity was calibrated on a hard mica surface. The adhesive forces were measured for the following configurations: (1) bare tips and normal

Personal Narrative The Cold Bucket Challenge - 1106 Words

Lying in bed, with the blankets tucked tightly around my head. Sleeping in a deep comatose, suddenly the alarm went off; squealing like my Company Commander woke us up. I quickly reached for the alarm clock and brought it down, towards my face, so I could attempt to see what time it was. I finally snapped out of my sleep induced haze, as if someone had dumped a bucket of cold water over my head, just like they were doing in the â€Å"Cold Bucket Challenge.† I thought to myself that I really had to out of my warm bed, to go pick up Jessica up from work. Quietly I laid there dreading getting up from the warm nest that I had built. Finally I cleared the cobwebs out of my sleep driven mind. And threw my legs over the side of the bed, standing there reaching for my glasses. I quickly got dressed, and opened up the curtain to take a look outside. As I stood there focusing my eyes. I snapped back into reality. I noticed that it had begun to snow, and man was it snowing har d. Shaking, my head, in disbelief, I walked into the kitchen to grab the broom. Stopping at the closet, I grabbed my coat and gloves. I thought to myself that this is could get real ugly. Carefully I opened the door, uncertain of what I was going to see. A cold blast of arctic air hit me, as if I stuck my head in a deep freezer. I carefully walked through the drifts, to get to the car. I walked extremely carefully so I wouldn’t take a nose dive into a friendly snowbank. Carefully I began to use theShow MoreRelatedStudy Guide Literary Terms7657 Words   |  31 PagesFlies provides a compelling allegory of human nature, illustrating the three sides of the psyche through its sharply-defined main characters. A form of extended metaphor, in which objects, persons, and actions in a narrative, are equated with the meanings that lie outside the narrative itself. 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Directing Act 5 Scene 2 Essay Example For Students

Directing Act 5 Scene 2 Essay I will be directing part of Act five scene two. This is the part where most of the main characters are all together and Petruchio shows all the other men that he has tamed Kate. All the men have a bet on whose wife is the most obedient.  I have chosen to set my piece in the present time because I want the modern day audience to sympathise with both Katherina and Petruchio. I am going to set this in a stately home because the men will be playing cards and the women will be talking in a nearby room. The servants will be also playing cards because Biondello and Gremio are also good friends of their masters. The house will belong to Lucentio. I have chose to use a stately home because it is the closest thing to the type of housing and the class the people in the original play were set in. I thought that although the stately home is the closest thing to the original, the modern day audience would still be able to relate to the characters and settings a lot easier. The men will be dressed in casual suits with a loosened tie, to create the effect of the upper class relaxing with a few friends. Kate will be wearing a long flowing dress to show that she is like an ordinary upper class lady instead of the more radical clothing she would have worn before the taming process. Biannca and the widow will also be wearing the same style of dress. The servants (Biondello and Gremio) would be wearing the traditional butler uniform.  I want them to realise that Petruchio has tamed Kate, but I want them to question whether or not Kate has lost her robust personality in the process. I want them to think that although Kate has been disciplined and is obeying Petruchios every word, that she still is her own person, but she now puts her view across in very different ways. I am going to direct my play in a way that will bring out the fact that Kate is still her own person, but a lot more sociable.  Baptista and all the other men think that Petruchio could not have tamed Kate because she was uncontrollable. So I would get Baptista to say this line   Now in good. shrew of all  In a patronising way. I would get him to say it in this way because Baptista doesnt think that Petruchio has succeeded.  Well, I say no. we will propose  Petruchio would say this in a confident manner, which the other gentlemen will act upon because they think that Kate has no chance of winning therefore they all place a wager for their own wife. Twenty crowns? Ill venture. Upon my wife  Again Petruchio would say this in a conclusive way to make lure the audience and his friends into thinking he must be foolish to bet such a large amount of money upon a bet he is in no doubt going to lose.  That will I. Go Biondello, bid your mistress come to me  Lucentio would say this in a boastful manner because he thinks his sweet fair Biannca will come to him at once. The audience are in total agreement with Lucentio, they also think Biannca will come. When Biondello exits he will go through a large oak door and you would hear him running down the hall. Son, Ill be your half Biannca comes  The actor would need to say this in a way that convinces the audience he is willing to give his money away because he is that assured that Lucentio will win the bet.  Ill have no halves; Ill bear it all myself.  Lucentio would say this in an arrogant way because he is that confident that he will win he wants all the rewards for himself.  Sir, my mistress. She cannot come.  Biondello would be out of breath after he had been running; he would then say this sentence in a very surprised way. Before he said this Lucentio would have a very smug grin on his face, but afterwards his face would drop.  How? Shes busy. Is that an answer?

Company Accounting for ASX Corporate Governance Principles

Question: Discuss about theCompany Accounting for ASX Corporate Governance Principles. Answer: Australias corporate governance and continuous disclosure framework is regarded the best in the world. This is the reason why it has also been adopted by various other jurisdictions. Moreover, the Corporations Law in Australia require organizations to keep the market informed of significant developments and there are negative consequences for those who fail. In relation to Getswift Ltd, it is notable that the acquisition of Pursuit Pty Ltd necessitates variations in its current corporate governance practices so that it can easily adhere to the ASX Corporate Governance Principles. In relation to structuring the Board of the company, it is notable that there are no committees established for the purpose of discharge of responsibilities. Furthermore, since acquisition of Pursuit Pty Ltd is a major task for Getswift, it is recommended that it facilitates proper provisions for the establishment of relevant committees like the nomination committee. The reason why establishment of such committee is crucial for the company can be attributed to the fact that it assists in examining the selection and appointment approaches of the company[1]. Furthermore, such committee must comprise of only non-executive directors who are responsible for reviewing Board succession measures, assessing performance of the Board and directors, and evaluating required and desirable competencies of such board members. Overall, with such recommendation, the company can be easily assisted in discharging its responsibilities in an effective way. Another crucial recommendation for the company in relation to compliance with the ASX Corporate Governance Principles is that it must make proper provisions for establishing an audit committee. Since the company has not designed an audit committee and it is overseen by the Board, it must be noted that integrity of financial reporting can only be attained through an audit committee[2]. Without an audit committee, Getswift cannot facilitate good corporate governance measure in the entire industry and may fail to achieve penetration into the market even by acquiring its major customer Pursuit Ltd. Furthermore, in relation to this, it must be noted that such establishment of audit committee must also have their own charter wherein the roles and responsibilities of committee members are clearly laid down. Moreover, if integrity of financial reporting is obtained through establishment of an audit committee, the company can easily comply with ASX Corporate Governance Framework[3]. Besides, such integrity is demanded by every stakeholder in the market and if Getswift does not consider such recommendation, it may fail to retain trust and confidence on the part of such stakeholders. The third recommendation to Getswift Ltd is that it must establish an efficient remuneration committee in its framework so that it can adhere to the requirements of ASX Corporate Governance Principles. The reason behind this can be attributed to the fact that the scale and size of the company has restricted it to frame a remuneration committee that will result in future complications after acquisition of Pursuit Ltd. Moreover, the roles and responsibilities of such committee are overseen by the Board of Getswift itself, thereby creating a scenario wherein it is becoming overburdened in nature. In relation to this, it is recommended to the company that it must design a proper remuneration committee so that the policies associated to remuneration are focused upon in an effective way. Besides, such committee must primarily comprise of non-executive directors of the company wherein majority are independent in nature[4]. However, if the board can manage the roles and responsibilities of t he Group, it can do so but there are various guidelines in relation to remuneration of executives and non-executive directors. In relation to this, it is observable that the company offers remuneration to its non-executive directors based on the level of remuneration paid to non-executive directors by organizations of similar size in the industry[5]. It is recommended that the company must remunerate its non-executive directors normally in the form of cash, superannuation contributions, non-cash benefits, or equity. Furthermore, such directors must not receive bonus payments or options by the Board. In addition, these directors must not be offered retirement benefits except for superannuation advantages. Therefore, the company must consider this recommendation of overseeing its fair and responsible strategy of remuneration for complying the objectives or requirements of ASX Corporate Governance Principles. Therefore, these recommendations can be taken into consideration that can allow Getswift comply with ASX requirements and effectively acquire its primary consumer named Pursuit Pty Ltd. Bibliography Fen Osler, Hampson. Turbulent Peace: the Challenges of Managing International Conflict, Washington, 2012. Kowalewski, Oskar. Corporate governance and corporate performance: financial crisis. Management Research Review 39, no. 1 (2016): 1494-1515. doi: 10.1108/MRR-12-2014-0287 Marc, Goergen. International Corporate Governance, Prentice Hall, 2012. Rachael Ajomboh, Ntongho,.Culture and corporate governance convergence. International Journal of Law and Management 58, no. 5 (2016): 523-544. doi: 10.1108/IJLMA-04-2015-0016 Thomas, Clarke. International Corporate Governance, New York: Routledge, 2010.

Herman Ebbinghaus Essays - Hermann Ebbinghaus, Cognition, Psychology

Herman Ebbinghaus During the late 1800's a new science was emerging in Europe. Psychology's roots can be traced back to Germany and a man by the name of William Wunt. Following Wunt other psychologists began emerging in different fields. Of these pioneers Herman Ebbinghaus was one, and his field of study was memory. He performed the first experiments in 1885 in Germany and the following is a background on the man and his field. Herman Ebbinghaus was born in 1850 in Germany and died there in 1909. He received his formal education at the Universities of Bonn, Halle, and Berlin (Gale, 1996). Ebbinghaus received degrees in philosophy and history from these universities (Gale, 1996). Ebbinghaus went on to teach at the Universities of Berlin, Poland, Breslaw and Halle (Gale, 1996). These experiences combined with later experiences with memory combine to give Ebbinghaus a curiosity about memory greater than most of his time. Memory can be defined as your amount of learning or your stored information. The process of storing and retreving information from the brain that is central to learning and thinking (Microsoft Encarta, [MSE], 1997). According to Myers (1998) memory is "any indication that learning has persisted over time". There are also four types of memory classified: recollection, recall, recognition, and relearning. Recollection is the reconstruction of facts based on clues that serve as reminders; recall is the active remembering of something from the past without help; recognition is the ability to identify previous stimuli as familiar; relearning is material that seems to be easier to remember than others as if it has been learned before (MSE, 1997). These four types of memory together help all people to remember anything from the states' capitals to your best friends birthday party from second grade. Some researchers say that there are specific sites dedicated to memory while others say that all the brain works together (MSE, 1997). There are tests to determine memory in individuals that Ebbinghaus Ebbinghaus 3 himself developed and will be discussed later. One test that does involve memory in a way would be the IQ test developed to test childrens level of intelligence which in turn depends on how much the child remembers. Ebbinghaus served in the Franco-Prussian War then seven years after that, decided to tutor in England, France and Berlin (Gale, 1996). It was during this time that Ebbinghaus became interested in memory and began to wonder how memory worked (Gale, 1996). In the journal of Physiological Psychology William Wunt said that a test on memory could not be performed (Gale, 1996). After reading this Ebbinghaus decided that he would try and test memory himself. Armed with his curiosity and his knowledge of memory from tutoring Ebbinghaus began the tests. He used the same mathematical treatment that Gustav Fechner used in Elements of Psychophysics to try and test memory experimentally (Gale, 1996). Ebbinghaus decided to be the subject and the experimenter in this test so he made a list of nonsense syllables that he would memorize (Myers, 1998). He crated 2,300 one syllable consonant-vowel-consonant combinations to make his study easier (Gale, 1996). He made words such as taz, bok, lef so that he could test the memorization rather than his previous knowledge of the words. He divided the material into lists that he memorized in different conditions (Gale, 1996). He measured them at night, in the day, when he was tired, just gotten up, etc. He recorded the average time it took him to memorize the lists perfectly then altared the test (Gale 1996). According to Gale (1996) he made observations about ther effects of such variables as speed, list length, and number of repetitions. Ebbinghaus also wanted to test long term and short term memory retention. He compared the time it took him to memorize any list once with the ammount of time it took him to memorize the same list again (Gale, 1996). He also measured immediate Ebbinghaus 4 memory showing that he remembered about six to eight items off his list after one look (Gale, 1996). Ebbinghaus in testing memory wanted to know how much he still knew from his lists later. According to Myers (1998) he would test himself on the same material thirty minutes to thirty days after his initial test. Using the mathematical methods mentioned earlier he came up with a retention curve showing how much of the information he was able to retrieve the next day. This figure can be seen on the attatched sheet, Figure 9.3. Ebbinghaus discovered that the longer he repeated the list on the

Consumer Behavior

Consumer Behavior Perception ContentsTitlePage no.Introduction01Model of consumer behavior02Physical influences within an individual03Motivation04Perception05Learning06Attitudes06Lifestyle06Social influences affect consumer behavior07Family07Social class07Reference group08Cultural factor affect consumer behavior08Culture08Subculture09Personal factors affect consumer behavior09Age and stage of life10Occupation10Life style10Individual are affected by the purchase situation11The-consumer adoption process11Factors influencing adoption process12Readiness to try new product and personal influence12Personal influence14Characteristics of the innovation14Organization's readiness to adopt innovation15Conclusion16Most economists assumes that consumers are economic buyers - people who know all the facts and logically compare choices in terms of costs and value received to get the greatest satisfaction from spending their time and money.This view assumes that economic needs guide most consumers' behavior.Samsung Galaxy Tab 1 0.1Note 10.1 was released on February 27, 2012 at Mobile World Congress in Barcelona, Spain. This tablet was released in Saudi Arabia, Germany and the United Arab Emirates on August 6, 2012. Note 10.1, including 16, 32 and 64 GB of internal storage's goal and 2 GB of DDR3 memory. On August 16, 2012, Note 10.1 was launched in the United States, South Korea and the United Kingdom.A reformation to Android 4.1 Jelly Bean was made available, either as an over-the-air update or through Samsung Kies. Samsung somehow has standardized the interface with its TouchWiz UX software. Some Samsung apps offer a "Split screen" function by which two apps will share the screen of the tablet in a time.ChatON has basic features such as automatic enrollment friends, chat, text multimedia. Advantage ChatOn is having an...

Mary Mount Universitys Student Code of Conduct Essay

Mary Mount Universitys Student Code of Conduct - Essay Example The Section 4 of Mary Mounts community code of conduct contains the special provision in part b which is very disturbing – it witchhunt on its perceived misconduct online. Students are cautioned that behavior conducted online, such as harassment or bullying via email, can subject them to University conduct action. Students must also be aware that blogs, web page entries on sites such as MySpace, Facebook, and Twitter and other similar online postings are in the public sphere and are not private. These postings can subject a student to allegations of conduct violations if evidence of policy violations is posted online. The University does not regularly search for this information but may take action if and when such information is brought to the attention of University officials. This is a chilling provision because, in effect, students cannot post or write anything that school officials might not like. It covers blogs, web page entries on sites such as MySpace, Facebook, and Twitter and other similar online postings under the license of bullying and harassment which would be difficult to interpret. For example, if a student rants about his or her disappointments which are typical among college students, and it was reported to school administrator as harassment, the student will be in trouble as what the special provision on Section 4 states. In effect, this provision dictates its student to only write and post nice things which are a gross violation of the freedom of speech as guaranteed by the Constitution in the First Amendment.